期刊
ACTA NEUROPATHOLOGICA COMMUNICATIONS
卷 4, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s40478-016-0380-x
关键词
Neurodegeneration; Alzheimer's disease; pyroglutamate Abeta; Drosophila
资金
- Max Planck Institute for the Biology of Ageing
- Alzheimer's Society
- Wellcome Trust
- Grants-in-Aid for Scientific Research [15K07092] Funding Source: KAKEN
Several species of beta-amyloid peptides (A beta) exist as a result of differential cleavage from amyloid precursor protein (APP) to yield various C-terminal A beta peptides. Several N-terminal modified A beta peptides have also been identified in Alzheimer's disease (AD) brains, the most common of which is pyroglutamate-modified A beta (A beta(pE3-42)). A beta(pE3-42) peptide has an increased propensity to aggregate, appears to accumulate in the brain before the appearance of clinical symptoms of AD, and precedes A beta(1-42) deposition. Moreover, in vitro studies have shown that A beta(pE3-42) can act as a seed for full length A beta(1-42). In this study, we characterized the Drosophila model of A beta(pE3-42) toxicity by expressing the peptide in specific sets of neurons using the GAL4-UAS system, and measuring different phenotypic outcomes. We found that A beta(pE3-42) peptide had an increased propensity to aggregate. Expression of A beta(pE3-42) in the neurons of adult flies led to behavioural dysfunction and shortened lifespan. Expression of A beta(pE3-42) constitutively in the eyes led to disorganised ommatidia, and activation of the c-Jun N-terminal kinase (JNK) signaling pathway. The eye disruption was almost completely rescued by co-expressing a candidate A beta degrading enzyme, neprilysin2. Furthermore, we found that neprilysin2 was capable of degrading A beta(pE3-42). Also, we tested the seeding hypothesis for A beta(pE3-42) in vivo, and measured its effect on A beta(1-42) levels. We found that A beta(1-42) levels were significantly increased when A beta(1-42) and A beta(pE3-42) peptides were co-expressed. Furthermore, we found that A beta(pE3-42) enhanced A beta(1-42) toxicity in vivo. Our findings implicate A beta(pE3-42) as an important source of toxicity in AD, and suggest that its specific degradation could be therapeutic.
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