Development of red genetically encoded biosensor for visualization of intracellular glucose dynamics

Glucose is the main source of energy for organisms, and it is important to understand the spatiotemporal dynamics of intracellular glucose. Single fluorescent protein-based glucose indicators, named Red Glifons'' have been developed that apply to live-cell and dual-color imaging. These indicators exhibited more than 3-fold increase in fluorescence intensity in the presence of 10 mM glucose. The two Red Glifons developed have different half-maximal effective concentration (EC50) values for glucose (300 mu M and 3,000 mu M) and are able to monitor a wide range of glucose dynamics. Red Glifon combined with green indicators allowing visualization of the interplay between glucose and ATP, lactate, or pyruvate. Glucose influx in the pharyngeal muscle of Caenorhabditis elegans, enteroendocrine cells, and human iPS cell-derived cardiac myocytes was observed using the Red Glifons. Thus these red glucose indicators serve as a multi-color imaging toolkit for investigating complex interactions in energy metabolism.

Automated summary

In this study, a new glucose indicator called Red Glifons was developed for real-time monitoring of intracellular glucose dynamics. These indicators exhibited high sensitivity and a wide range of applications, making them valuable tools for studying complex interactions in energy metabolism.