期刊
EBIOMEDICINE
卷 9, 期 -, 页码 228-237出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ebiom.2016.06.016
关键词
Real-time PCR; Line probe assay; Mycobacterium tuberculosis; Nontuberculous mycobacteria; Drug susceptibility
资金
- University of Zurich, Switzerland
- Federal Office of Public Health, Switzerland
Molecular assays have not yet been able to replace time-consuming culture-based methods in clinical mycobacteriology. Using 6875 clinical samples and a study period of 35 months we evaluated the use of PCR-based assays to establish a diagnostic workflow with a fast time-to-result of 1-2 days, for 1. detection of Mycobacterium tuberculosis complex (MTB), 2. detection and identification of nontuberculous mycobacteria (NTM), and 3. identification of drug susceptible MTB. MTB molecular-based detection and culture gave concordant results for 97.7% of the specimens. NTM PCR-based detection and culture gave concordant results for 97.0% of the specimens. Defining specimens on the basis of combined laboratory data as true positives or negatives with discrepant results resolved by clinical chart reviews, we calculated sensitivity, specificity, PPV and NPV for PCR-based MTB detection as 84.7%, 100%, 100%, and 98.7%; the corresponding values for culture-based MTB detection were 86.3%, 100%, 100%, and 98.8%. PCR-based detection of NTM had a sensitivity of 84.7% compared to 78.0% of that of culture-based NTM detection. Molecular drug susceptibility testing (DST) by line-probe assay was found to predict phenotypic DST results in MTB with excellent accuracy. Our findings suggest a diagnostic algorithmto largely replace lengthy culture-based techniques by rapid molecular- based methods. (C) 2016 The Authors. Published by Elsevier B.V.
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