4.8 Article

In vivo imaging of the human eye using a 2-photon-excited fluorescence scanning laser ophthalmoscope

期刊

JOURNAL OF CLINICAL INVESTIGATION
卷 132, 期 2, 页码 -

出版社

AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI154218

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资金

  1. European Union under the European Regional Development Fund [MAB/2019/12, POIR.04.04.00-00-3D47/16-00]
  2. Foundation for Polish Science within the First TEAM program - European Union under the European Regional Development Fund [TEAM/2017-4/39]
  3. NIH [EY009339, EY027283]
  4. Research to Prevent Blindness
  5. National Science Centre [2016/23/B/ST2/00752]
  6. Polish National Agency for Academic Exchange [PPN/PPO/2018/1/00082]
  7. Polish Ministry of Science and Higher Education (2016-2019 int.)

向作者/读者索取更多资源

Researchers have developed a new method for noninvasive metabolic assessment of the human retina. Using a compact 2-photon-excited fluorescence scanning laser ophthalmoscope and near-infrared light, they were able to obtain spectrally resolved images of the human retina. This approach provides the possibility of monitoring eye diseases in the earliest stages before structural damage occurs to the retina.
BACKGROUND. Noninvasive assessment of metabolic processes that sustain regeneration of human retinal visual pigments (visual cycle) is essential to improve ophthalmic diagnostics and to accelerate development of new treatments to counter retinal diseases. Fluorescent vitamin A derivatives, which are the chemical intermediates of these processes, are highly sensitive to UV light; thus, safe analyses of these processes in humans are currently beyond the reach of even the most modern ocular imaging modalities. METHODS. We present a compact, 2-photon-excited fluorescence scanning laser ophthalmoscope and spectrally resolved images of the human retina based on 2-photon excitation (TPE) with near-infrared light. A custom Er:fiber laser with integrated pulse selection, along with intelligent postprocessing of data, enables excitation with low laser power and precise measurement of weak signals. RESULTS. We demonstrate spectrally resolved TPE fundus images of human subjects. Comparison of TPE data between human and mouse models of retinal diseases revealed similarity with mouse models that rapidly accumulate bisretinoid condensation products. Thus, visual cycle intermediates and toxic byproducts of this metabolic pathway can be measured and quantified by TPE imaging. CONCLUSION. Our work establishes a TPE instrument and measurement method for noninvasive metabolic assessment of the human retina. This approach opens the possibility for monitoring eye diseases in the earliest stages before structural damage to the retina occurs.

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