期刊
HORTICULTURAE
卷 8, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/horticulturae8010013
关键词
black spot; 'CuiXiang'; kiwifruit; internal transcribed spacer sequencing; transcriptome analysis
类别
资金
- Primary Research and Development Plan of Ningxia Hui Autonomous Region [2021BBF0214]
- Introduction of Domestic Doctoral Programs in Shaanxi Province [16-18]
- Chinese Universities Scientific Fund [2452019018]
This study evaluated and analyzed the pathogenic microorganisms of kiwifruit black spot disease, and revealed the mechanism and influencing factors of the disease, as well as identified the potential pathogenic fungi causing the disease.
Kiwifruit black spot disease has become increasingly widespread in many 'CuiXiang' kiwifruit plantings regions. This research was aimed at the pathogenic microorganisms of black spot of the 'CuiXiang' cultivar. Physiological, morphological and transcriptional characteristics between black spot fruit and healthy fruits were evaluated. Then, it applied a high-throughput internal transcribed spacer (ITS) sequencing to analyze the black spot disease microbial community. The cell structure showed that mycelium was attached to the surface of the kiwifruit through black spot, and that consequently the mitochondria were damaged, starch particles were reduced, and shelf life was shortened. Transcriptome revealed that different genes in kiwifruit with black spot disease were involved in cell wall modification, pathogen perception, and signal transduction. ITS sequencing results described the disease-causing fungi and found that the microbial diversity of black spot-diseased fruit was lower than that of healthy fruit. We predict that candidate pathogenic fungi Cladosporium cladosporioides, Diaporthe phaseolorum, Alternaria alternata, and Trichothecium roseum may cause black spot. This study was to explore the pathogenic fungal community of 'CuiXiang' kiwifruit black spot disease and to provide essential information for field prevention.
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