期刊
SCIENCE ADVANCES
卷 2, 期 11, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.1601266
关键词
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资金
- excellence initiative of the German federal government [DFG EXC-1028-CEPLAS, EXC 294-BIOSS, GSC 4-SGBM]
- excellence initiative of the German state government [DFG EXC-1028-CEPLAS, EXC 294-BIOSS, GSC 4-SGBM]
- King Abdullah University of Science and Technology (KAUST)
Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.
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