期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 86, 期 3, 页码 313-320出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbab219
关键词
Endonuclease V; 1-methylinosine; inosine; RNA; Archaea
类别
资金
- 2019 Inamori Research Grants Program
This study explored the biochemical properties of EndoV in the hyperthermophilic archaeon Thermococcus kodakarensis and found that it has a strong preference for RNA and selectively acts on inosine. These findings suggest a potential role of archaeal EndoV in the regulation of inosine-containing RNA.
Endonuclease V (EndoV) is an inosine-specific endonuclease which is highly conserved in all domains of life: Bacteria, Archaea, and Eukarya; and, therefore, may play an important role in nucleic acid processes. It is currently thought that bacterial EndoVs are involved in DNA repair, while eukaryotic EndoVs are involved in RNA editing based on the differences in substrate preferences. However, the role of EndoV proteins, particularly in the archaeal domain, is still poorly understood. Here, we explored the biochemical properties of EndoV from the hyperthermophilic archaeon Thermococcus kodakarensis (TkoEndoV). We show that TkoEndoV has a strong preference for RNA over DNA. Further, we synthesized 1-methylinosine-containing RNA that is a simple T psi C loop mimic of archaeal tRNA and found that TkoEndoV discriminates between 1-methylinosine and inosine, and selectively acts on inosine. Our findings suggest a potential role of archaeal EndoV in the regulation of inosine-containing RNA.
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