期刊
SENSORS AND ACTUATORS REPORTS
卷 4, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.snr.2022.100080
关键词
Sensor; Neurotransmitter; C8H11NO2; Carbon spheres; SH-SY5Y cells
类别
资金
- Suzhou University of Science and Technology
- Southwest University
- Third Military Medical University
This study develops an electrochemical biosensor based on hollow carbon spheres, aptamer, and methylene blue (MB) for monitoring dopamine release from Parkinson's disease model cells. The sensor exhibits a low detection limit, wide detection range, high sensitivity, and good selectivity, surpassing previously reported aptamer sensors. By intercalating MB, the sensor provides two reaction sites for dopamine absorption, doubling the detection sensitivity. The sensor is successfully used to monitor dopamine release from living cells, confirming the effect of 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine on dopamine synthesis and demonstrating its potential for real-time detection. This work introduces a new approach to enhance detection sensitivity by increasing aptamer reaction sites.
Dopamine plays a critical role in some fatal diseases including Parkinson's disease, schizophrenia, Tourette syndrome and HIV infection studies but its detection sensitivity demands further improvement. Aptamer-based biosensor has good selectivity to detect biologically active substances; however, it is very challenging to achieve high sensitivities for practical applications. Here an electrochemical biosensor is constructed by hollow carbon spheres, aptamer and methylene blue (MB) to monitor dopamine released from Parkinson's disease model (SHSY5Y) cells with a low detection limit, wide detection range, high sensitivity and good selectivity for much better performance than reported aptamer sensors. Investigation discovers that MB intercalation in aptamers can offer two sites to absorb two dopamine through pi-pi interaction instead of one DA absorption on an aptamer alone, thereby doubling the detection sensitivity. The sensor was further used to monitor dopamine released from SH-SY5Y cells, clearly confirming the effect of 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine on dopamine synthesis in SH-SY5Y cells and demonstrating the great potential of the sensor in real-time detect dopamine released from living cells. This work renders a new avenue to amplify the detection sensitivity by intercalating redox small molecule to increase the aptamer reaction sites while bridging the interfacial electron transfer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据