期刊
GELS
卷 8, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/gels8020084
关键词
gelatin methacrylate; 3D culture; Schwann cells; neurotrophic factors; nerve regeneration
资金
- National Key Research and Development Program of China [2018YFA0703000]
- National Natural Science Foundation of China [52075482]
- Zhejiang Medical Science and Technology Project [2018KY683, 2019KY569]
This study investigated the co-culture of Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on a 50% degree of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. PC-12 cells showed enhanced cell proliferation and axonal extension in the presence of neurotrophic factors secreted by SCs. A 5% GelMA scaffold was found to better support SCs proliferation, viability, and myelination compared to a 10% GelMA scaffold.
Peripheral nerve injuries cause different degrees of nerve palsy and function loss. Due to the limitations of autografts, nerve tissue engineering (TE) scaffolds incorporated with various neurotrophic factors and cells have been investigated to promote nerve regeneration. However, the molecular mechanism is still poorly understood. In this study, we co-cultured Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on 50% degrees of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. The SCs were encapsulated within the GelMA, and PC-12 cells were on the surface. A 5% GelMA was used as the co-culture scaffold since it better supports SCs proliferation, viability, and myelination and promotes higher neurotrophic factors secretion than 10% GelMA. In the co-culture, PC-12 cells demonstrated a higher cell proliferation rate and axonal extension than culturing without SCs, indicating that the secretion of neurotrophic factors from SCs can stimulate PC-12 growth and axonal outgrowth. The mRNA level for neurotrophic factors of SCs in 5% GelMA was further evaluated. We found significant upregulation when compared with a 2D culture, which suggested that this co-culture system could be a potential scaffold to investigate the mechanism of how SCs affect neuronal behaviors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据