Genome-resolved metagenomics provides insights into the functional complexity of microbial mats in Blue Holes, Shark Bay

The present study describes for the first time the community composition and functional potential of the microbial mats found in the supratidal, gypsum-rich and hypersaline region of Blue Holes, Shark Bay. This was achieved via high-throughput metagenomic sequencing of total mat community DNA and complementary analyses using hyperspectral confocal microscopy. Mat communities were dominated by Proteobacteria (29%), followed by Bacteroidetes/Chlorobi group (11%) and Planctomycetes (10%). These mats were found to also harbour a diverse community of potentially novel microorganisms, including members from the DPANN, Asgard archaea and candidate phyla radiation, with highest diversity found in the lower regions (similar to 14-20 mm depth) of the mat. In addition to pathways for major metabolic cycles, a range of putative rhodopsins with previously uncharacterized motifs and functions were identified along with heliorhodopsins and putative schizorhodopsins. Critical microbial interactions were also inferred, and from 117 medium- to high-quality metagenome-assembled genomes, viral defence mechanisms (CRISPR, BREX and DISARM), elemental transport, osmoprotection, heavy metal resistance and UV resistance were also detected. These analyses have provided a greater understanding of these distinct mat systems in Shark Bay, including key insights into adaptive responses and proposing that photoheterotrophy may be an important lifestyle in Blue Holes. Cross section of microbial mat from Blue Holes, Shark Bay, Australia.

Automated summary

This study provides the first description of the community composition and functional potential of microbial mats found in the gypsum-rich and hypersaline region of Blue Holes, Shark Bay. The dominant groups were found to be Proteobacteria, Bacteroidetes/Chlorobi, and Planctomycetes, with a diverse community of potentially novel microorganisms also present. The study identified important microbial interactions and functional pathways in these mats.