期刊
BIOMATERIALS SCIENCE
卷 10, 期 7, 页码 1803-1811出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1bm01722e
关键词
-
资金
- National Key Research and Development Project of Stem Cell and Transformation Research [2019YFA0112100, 2019YFA0112102]
- National Natural Science Foundation of China [81973252, 81803451, 81620108028, 81871403]
- Ten-thousand Talents Program of Zhejiang Province [2018R52049]
- Key Research and Development Program of Zhejiang Province [2019C03014]
Functional recovery after traumatic spinal cord injury (SCI) remains a difficult problem to solve due to the limited efficacy of current clinical treatment strategies. In this research, exosomes were engineered through hypoxia stimulation to mesenchymal stem cells and transplanted into the injured spinal cord via encapsulation in a peptide-modified adhesive hydrogel for promoting angiogenesis in SCI therapy. Significant angiogenesis and functional recovery after injury were demonstrated both in vitro and in vivo, indicating the immense potential of hydrogel-encapsulated hypoxia-stimulated exosomes in treating central nervous system trauma and other ischemia diseases.
Due to the limited efficacy of current clinical treatment strategies, functional recovery after traumatic spinal cord injury (SCI) remains a knotty problem to be solved. Apart from anti-inflammation and cell replenishing treatments, accumulating evidence implies that promoting angiogenesis would also potentially benefit tissue regeneration after SCI. In this research, inspired by the role of exosomes in cell-cell communication and exosomal alteration resulting from cells under stress, exosomes were engineered through hypoxia stimulation to mesenchymal stem cells and were proposed as an alternative for promoting angiogenesis in SCI therapy. Hypoxia-stimulated exosomes (hypo-Exo) were transplanted into the injured spinal cord via encapsulation in a peptide-modified adhesive hydrogel for pro-angiogenic therapy of SCI. The adhesive peptide PPFLMLLKGSTR-modified hyaluronic acid hydrogel replenished the spinal cavity caused by SCI and achieved the local delivery of exosomes. The hypoxia-inducible factor 1-alpha content in hypo-Exo was significantly increased, resulting in the overexpression of vascular endothelial growth factor in the endothelial cells surrounding the transplant system. Ultimately, prominent angiogenesis and functional recovery after injury were demonstrated both in vitro and in vivo, indicating the immense potential of hydrogel-encapsulated hypo-Exo in treating central nervous system trauma and other ischemia diseases.
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