期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 86, 期 4, 页码 464-475出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbac015
关键词
lacto-N-biosidase; glycoside hydrolase family 136; human milk oligosaccharides; lacto-N-biose I; human gut microbiota
类别
资金
- Japan Society for the Promotion of Science under KAKENHI [18K14388, 16J09251]
- The Japan Science Society
- Grants-in-Aid for Scientific Research [18K14388, 16J09251] Funding Source: KAKEN
Glycoside hydrolase family 136 (GH136) was established based on the discovery and structural analysis of lacto-N-biosidase (LNBase) from Bifidobacterium longum subsp. longum JCM1217 (BlLnbX). Homologous genes of BlLnbX are widely found in human gut bacteria and monkey Bifidobacterium spp. Other bacterial strains like Bifidobacterium saguini, Tyzzerella nexilis, and Ruminococcus lactaris also exhibit LNBase activity. Crystal structures of LNBases from B. saguini and T. nexilis were determined in complex with lacto-N-biose I, showing a conserved fold and catalytic residues.
Glycoside hydrolase family 136 (GH136) was established after the discovery and structural analysis of lacto-N-biosidase (LNBase) from the infant gut bacterium Bifidobacterium longum subsp. longum JCM1217 (BlLnbX). Homologous genes of BlLnbX are widely distributed in the genomes of human gut bacteria and monkey Bifidobacterium spp., although only 2 crystal structures were reported in the GH136 family. Cell suspensions of Bifidobacterium saguini, Tyzzerella nexilis, and Ruminococcus lactaris exhibited the LNBase activity. Recombinant LNBases of these 3 species were functionally expressed with their specific chaperones in Escherichia coli, and their kinetic parameters against p-nitrophenol substrates were determined. The crystal structures of the LNBases from B. saguini and T. nexilis in complex with lacto-N-biose I were determined at 2.51 and 1.92 angstrom resolutions, respectively. These structures conserve a beta-helix fold characteristic of GH136 and the catalytic residues, but they lack the metal ions that were present in BlLnbX.
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