Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis

In this protocol, we describe global proteome profiling for the respiratory specimen of COVID-19 patients, patients suspected with COVID-19, and H1N1 patients. In this protocol, details for identifying host, viral, or bacterial proteome (Meta-proteome) are provided. Major steps of the protocol include virus inactivation, protein quantification and digestion, desalting of peptides, high-resolution mass spectrometry (HRMS)-based analysis, and downstream bioinformatics analysis. For complete details on the use and execution of this profile, please refer to Maras et al. (2021).

Automated summary

This paper presents a protocol for global proteome profiling of respiratory specimens from COVID-19 patients, patients suspected with COVID-19, and H1N1 patients. The method includes virus inactivation, protein quantification and digestion, desalting of peptides, high-resolution mass spectrometry-based analysis, and downstream bioinformatics analysis.