Phase coherent excitation of SABRE permits simultaneous hyperpolarization of multiple targets at high magnetic field

Hyperpolarization methods in magnetic resonance overcome sensitivity limitations, especially for low-gamma nuclei such as C-13 and N-15. Signal Amplification By Reversible Exchange (SABRE) and extended SABRE (X-SABRE) are efficient and low-cost methods for generating large polarizations on a variety of nuclei, but they most commonly use low magnetic fields (mu T-mT). High field approaches, where hyperpolarization is generated directly in the spectrometer, are potentially much more convenient but have been limited to selectively hyperpolarize single targets. Here we introduce a new pulse sequence-based approach that affords broadband excitation of SABRE hyperpolarization at high magnetic fields without having to tailor pulse sequence parameters to specific targets. This permits simultaneous hyperpolarization of multiple targets for the first time at high field and offers a direct approach to integration of high-field SABRE hyperpolarization into routine NMR applications, such as NMR-based metabonomics and biomolecular NMR.

Automated summary

Hyperpolarization methods in magnetic resonance, such as SABRE and X-SABRE, have the potential to generate large polarizations on multiple targets at high magnetic fields. However, previous methods have been limited to selectively hyperpolarizing single targets. In this study, a new pulse sequence-based approach is introduced, allowing for broadband excitation of SABRE hyperpolarization at high fields, which enables simultaneous hyperpolarization of multiple targets.