期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 20, 期 13, 页码 2636-2642出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ob00328g
关键词
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资金
- JSPS [JP20H05620]
- MEXT [JP17H06402]
- KAKENHI from JSPS [18J21506, 19K22330]
- Grants-in-Aid for Scientific Research [19K22330, 18J21506] Funding Source: KAKEN
2-Azahypoxanthine (AHX) is a compound that can induce fairy ring formation. It has been found in numerous plants and mushrooms. By studying the fungus L. sordida, it was discovered that reactive nitrogen species (RNS), derived from nitric oxide (NO) produced by NO synthase (NOS), are the key nitrogen sources for the formation of the 1,2,3-triazine moiety present in AHX. It was also found that RNS are involved in the conversion of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5 '-monophosphate (AICAR) to AHX-ribotide (AHXR), suggesting the existence of a novel biosynthetic route in the fungus. These findings highlight the physiological role of NOS in the biosynthesis of AHX and other natural products containing a nitrogen-nitrogen bond.
2-Azahypoxanthine (AHX) was first isolated from the culture broth of the fungus Lepista sordida as a fairy ring-inducing compound. It has since been found that a large number of plants and mushrooms produce AHX endogenously and that AHX has beneficial effects on plant growth. The AHX molecule has an unusual, nitrogen-rich 1,2,3-triazine moiety of unknown biosynthetic origin. Here, we establish the biosynthetic pathway for AHX formation in L. sordida. Our results reveal that the key nitrogen sources that are responsible for the 1,2,3-triazine formation are reactive nitrogen species (RNS), which are derived from nitric oxide (NO) produced by NO synthase (NOS). Furthermore, RNS are also involved in the biochemical conversion of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5 '-monophosphate (AICAR) to AHX-ribotide (AHXR), suggesting that a novel biosynthetic route that produces AHX exists in the fungus. These findings demonstrate a physiological role for NOS in AHX biosynthesis as well as in biosynthesis of other natural products containing a nitrogen-nitrogen bond.
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