期刊
ACS SENSORS
卷 2, 期 1, 页码 52-56出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00651
关键词
upconversion nanoparticles; DNA; graphene oxide; biomarkers; biosensor; mRNA
资金
- University of Southampton
- EPSRC via a DTA studentship
- Biotechnology and Biological Sciences Research Council [BB/N021150/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1371608] Funding Source: researchfish
- BBSRC [BB/J001694/1, BB/N021150/1, BB/J001694/2] Funding Source: UKRI
The development of new sensors for the accurate detection of biomarkers in biological fluids is of utmost importance for the early diagnosis of diseases. Next to advanced laboratory techniques, there is a need for relatively simple methods which can significantly broaden the availability of diagnostic capability. Here, we demonstrate the successful application of a sensor platform based on graphene oxide and upconversion nanoparticles (NPs) for the specific detection of mRNA-related oligonucleotide markers in complex biological fluids. The combination of near-infrared light upconversion with low-background photon counting readout enables reliable detection of low quantities of small oligonucleotide sequences in the femtomolar range. We demonstrate the successful detection of analytes relevant to mRNAs present in Alzheimer's disease as well as prostate cancer in human blood serum. The high performance and relative simplicity of the upconversion NP-graphene sensor platform enables new opportunities in early diagnosis based on specific detection of oligonucleotide sequences in complex environments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据