Quantitative Label-Free Listeria Analysis Based On Aptamer Modified Nanoporous Sensor

In this Letter, we proposed a nanoporous sensor to realize a quick and sensitive detection of Listeria monocytogenes with the application of porous anodic aluminum oxide membrane combined with a specific aptamer. On the basis of electrochemical detection, it can be known that the reduction current response upon the diffusion flux of electrochemical probe is affected by the charge repulsion and steric blockage of Listeria monocytogenes. A rapid detection can be achieved within 10 min, with a high sensitivity of 100 CFU/mL and a good linear range between 100 to 1250 CFU/mL. Moreover, it has a high specificity for Listeria monocytogenes even exposed in 10(8) CFU/mL Staphylococcus aureus or Escherichia coli.