3.8 Article

Relevance of Nutrient-Sensing in the Pathogenesis of Trichophyton rubrum and Trichophyton interdigitale

期刊

FRONTIERS IN FUNGAL BIOLOGY
卷 3, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/ffunb.2022.858968

关键词

Trichophyton; tricarboxylic acid cycle; glyoxylate cycle; methylcitrate cycle; cellular cycle; subtilisin

类别

资金

  1. FAPESP [2019/22596-9, 2018/11319-1, 2009/08411-4, 2009/15426-8]
  2. CNPq [305797/2017-4, 304989/2017-7]
  3. CAPES [001]
  4. FAEPA of the HCFMRP-USP

向作者/读者索取更多资源

The growth and development of organisms depend on nutrient availability. This study compares the expression profile of genes involved in metabolism, cell cycle control, and proteases in two Trichophyton species, Trichophyton rubrum and Trichophyton interdigitale, in response to nutrient and pH variations. The results reveal species-specific responses and suggest that T. interdigitale has improved adaptation to keratin compared to T. rubrum.
The growth and development of organisms depend on nutrient availability. Dermatophytes must sense nutrient levels and adapt to the host environment to colonize human and animal keratinized tissues. Owing to the clinical importance of the Trichophyton genus, this study compared the expression profile of genes involved in metabolism, cell cycle control, and proteases in two Trichophyton species, Trichophyton rubrum, and Trichophyton interdigitale, in response to nutrients and environmental pH. In addition, we evaluated the activity of enzymes in the tricarboxylic acid, glyoxylate, and methylcitrate cycles. Moreover, the effects of interruption of the transcription factor pacC on T. interdigitale in the same conditions as for the wild-type strain were determined. Our analyses revealed specific responses in each species to the nutritional and pH variation. An improved adaptation of T. interdigitale to keratin was observed, compared with that of T. rubrum. T. rubrum growth in buffered keratin media indicated pH 8.0 as an optimal pH condition for metabolic activity, which differed from that for T. interdigitale. Tricarboxylic acid components in T. rubrum showed increased enzymatic activity and transcript accumulation. In T. interdigitale, a higher activity of enzymes in glyoxylate and methylcitrate cycles was observed, with no direct correlation to the transcriptional profile. T. interdigitale fungal metabolism suggests the requirement of anaplerotic pathways in the late cultivation period. The identified differences between T. rubrum and T. interdigitale may represent determinants for adaptation to the host and the incidence of infection with each species.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

3.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据