期刊
METALLOMICS
卷 14, 期 3, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/mtomcs/mfac015
关键词
inductively coupled plasma-mass spectrometry; single cell ICP-MS; copper incorporation; nanoparticles; antibiotics
资金
- government of Asturias through the Consejeria de Empleo, Industria y Turismo del Principado de Asturias - FEDER funds [FC-GRUPIN-IDI/2018/000242, FC-GRUPIN-IDI/2018/000120]
- Spanish Ministry of Economy, Industry and Competitiveness (MINECO) [PID2019-104334RB-I00, RTI2018-094605-B-I00]
- Ministerio de Ciencia, Innovacion Universidades/Agencia Estatal de Investigacion/Fondo Europeo de Desarrollo Regional [RTI2018-093978-B-I00]
- Instituto de Salud Carlos III for the postdoctoral Sara Borrell contract [CD19/00249]
This study investigates the incorporation and storage form of copper ions in Streptomyces and reveals the correlation between copper nanoparticles and antibiotic production, suggesting their role in modulating secondary metabolism and preventing copper toxicity.
Copper modulates secondary metabolism in Streptomyces. Although the cytosolic copper concentration is controlled by several chaperones and transporters, the formation of copper nanoparticles (NPs) and its relation to the antibiotic production has never been established in the model Streptomyces coelicolor. In this work, state-of-the-art analytical tools are used to evaluate the incorporation of copper in individual spores of S. coelicolor at different exposure concentrations (40, 80, and 160 mu M Cu). Among them, the use of single cell-inductively coupled plasma-mass spectrometry revealed incorporation levels in the range of 2 to 2.5 fg/spore (median) increasing up to 4.75 fg/spore at the upper exposure concentrations. The copper storage within the spores in the form of NPs was evaluated using a combination of single particle-inductively coupled plasma-mass spectrometry and transmission electron microscopy. The obtained data confirmed the presence of NPs in the range of 8 to 40 (mean size 21 nm) inside S. coelicolor spores. The presence of the NPs was correlated with the actinorhodin production in liquid non-sporulating cultures amended with up to 80 mu M Cu. However, further increase to 160 mu M Cu, yielded to a significant decrease in antibiotic production. Secondary metabolism is activated under stressful conditions and cytosolic copper seems to be one of the signals triggering antibiotic production. Particularly, NP formation might contribute to modulate the secondary metabolism and prevent for copper toxicity. This work describes, for first time, the formation of endogenous copper NPs in S. coelicolor and reveals their correlation with the secondary metabolism.
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