Optimisation of Xylanase-Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 degrees C, pH of 8.38, inoculum size of 4% (w/v) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model's validity. The optimised CCD model had a 1.34-fold, 159 +/- 6 U/mL greater xylanase and 5.96-fold, 205 +/- 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase-pectinase resulted in a ratio of 1:1.3.

Automated summary

An effective statistical tool, response surface methodology (RSM) using central composite design (CCD), was used to increase the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 in submerged fermentation (SmF). The optimized CCD model resulted in significantly higher xylanase and pectinase production compared to the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase and pectinase showed a ratio of 1:1.3.