期刊
JOURNAL OF DEVELOPMENTAL BIOLOGY
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/jdb10010009
关键词
Hox genes; Hoxa2; Hoxa5; cranial neural crest cells; gain-of-function; craniofacial morphogenesis; pinna; middle ear; hyoid; homeotic transformation; hypoplasia
资金
- Japan Society for the Promotion of Science fellowships [14j04690, 995333]
- Swiss National Science Foundation [31003A_149573, 31003A_175776]
- Novartis Research Foundation
- European Research Council under the European Union [810111-EpiCrest2Reg]
- Swiss National Science Foundation (SNF) [31003A_175776] Funding Source: Swiss National Science Foundation (SNF)
- Grants-in-Aid for Scientific Research [14J04690] Funding Source: KAKEN
This study reveals the importance of providing correct positional identity and patterning information to cranial neural crest cells (CNCCs) in vertebrate craniofacial morphogenesis. The study found that the levels of Hoxa2 overexpression play a crucial role in CNCC development, with different levels leading to different morphological outcomes.
Providing appropriate positional identity and patterning information to distinct rostrocaudal subpopulations of cranial neural crest cells (CNCCs) is central to vertebrate craniofacial morphogenesis. Hox genes are not expressed in frontonasal and first pharyngeal arch (PA1) CNCCs, whereas a single Hox gene, Hoxa2, is necessary to provide patterning information to second pharyngeal arch (PA2) CNCCs. In frog, chick and mouse embryos, ectopic expression of Hoxa2 in Hox-negative CNCCs induced hypoplastic phenotypes of CNCC derivatives of variable severity, associated or not with homeotic transformation of a subset of PA1 structures into a PA2-like identity. Whether these different morphological outcomes are directly related to distinct Hoxa2 overexpression levels is unknown. To address this issue, we selectively induced Hoxa2 overexpression in mouse CNCCs, using a panel of mouse lines expressing different Hoxa2 ectopic expression levels, including a newly generated Hoxa2 knocked-in mouse line. While ectopic Hoxa2 expression at only 60% of its physiological levels was sufficient for pinna duplication, ectopic Hoxa2 expression at 100% of its normal level was required for complete homeotic repatterning of a subset of PA1 skeletal elements into a duplicated set of PA2-like elements. On the other hand, ectopic Hoxa2 overexpression at non-physiological levels (200% of normal levels) led to an almost complete loss of craniofacial skeletal structures. Moreover, ectopic Hoxa5 overexpression in CNCCs, while also resulting in severe craniofacial defects, did not induce homeotic changes of PA1-derived CNCCs, indicating Hoxa2 specificity in repatterning a subset of Hox-negative CNCCs. These results reconcile some discrepancies in previously published experiments and indicate that distinct subpopulations of CNCCs are differentially sensitive to ectopic levels of Hox expression.
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