期刊
FERMENTATION-BASEL
卷 8, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/fermentation8030096
关键词
acid tolerance; lactic acid bacterium (LAB); adaptive evolution; metabolic engineering; wheat straw
资金
- [31961133006]
- [21978083]
This study aimed to enhance the acid tolerance of an engineered Pediococcus acidilactici strain using metabolic modification and adaptive evolution approaches. While overexpression of certain genes did not show significant improvement, low pH adaptive evolution resulted in clear enhancement. However, further decrease in pH led to a dramatic reduction in L-lactic acid generation.
Acid tolerance of the lactic acid bacterium (LAB) is crucially important for the production of free lactic acid as a chemical monomer by simplified purification steps. This study conducts both metabolic modification and adaptive evolution approaches on increasing the acid tolerance of an engineered Pediococcus acidilactici strain. The overexpression of the genes encoding lactate dehydrogenase, recombinase, chaperone, glutathione and ATPase did not show the observable changes in acid tolerance. On the other hand, the low pH adaptive evolution showed clear improvement. The L-lactic acid generation and cell viability of the adaptively evolved P. acidilactici were doubled at low pH up to 4.0 when wheat straw was used as carbohydrate feedstock. However, the further decrease in pH value close to the pKa (3.86) of lactic acid led to a dramatic reduction in L-lactic acid generation. This result shows a partially successful approach on improving the acid tolerance of the lactic acid bacterium P. acidilactici.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据