4.7 Article

RAA Enzyme Is a New Family of Class A Extended-Spectrum β-Lactamase from Riemerella anatipestifer Strain RCAD0122

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出版社

AMER SOC MICROBIOLOGY

关键词

Riemerella anatipestifer; new family; beta-lactamases; RAA

资金

  1. National Natural Science Foundation of China [31902340]
  2. Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0532]

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Whole-genome sequencing of Riemerella anatipestifer isolate RCAD0122 identified a novel ESBL gene, bla(RAA-1), which encoded a class A extended-spectrum beta-lactamase with low sequence identity to known beta-lactamases. The purified RAA-1 showed ESBL-like hydrolysis activity, and the gene could be transferred to a homologous strain through natural transformation. However, epidemiological study revealed the gene is not currently prevalent.
Whole-genome sequencing of Riemerella anatipestifer isolate RCAD0122 revealed a chromosomally located beta-lactamase gene, bla(RAA-1), which encoded a novel class A extended-spectrum beta-lactamase (ESBL), RAA-1. RAA-1 shared <= 65% amino acid sequence identity with other characterized beta-lactamases. The kinetic assay of native purified RAA-1 revealed ESBL-like hydrolysis activity. Furthermore, bla(RAA)(-1), could be transferred to a homologous strain by natural transformation. However, an epidemiological study showed that the bla(RAA)(-1) gene is not prevalent currently.

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