3.8 Article

High Prevalence of blaCTX-M in Fecal Commensal Escherichia coli from Healthy Children

期刊

INFECTION AND CHEMOTHERAPY
卷 54, 期 1, 页码 59-69

出版社

KOREAN SOC ANTIMICROBIAL THERAPY
DOI: 10.3947/ic.2021.0102

关键词

Antibiotic-resistance; Commensal; Extended-spectrum beta-lactamases; Escherichia coli; Healthy carriers

资金

  1. Thrasher Research Fund, USA
  2. Fondo Nacional de Desarrollo Cientifico, Tecnologico y de Innovacion Tecnologica (FONDECYT-Peru) en el marco del Proyectode Mejoramiento y Ampliacion de los Servicios del Sistema Nacional de Ciencia, Tecnologia e Innovacion Tecnologica [08-2019-FONDECYT-BM-INC-INV]

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This study demonstrates a high prevalence of ESBL-carriers among healthy children living in a rural area of Peru, highlighting the need for continuous surveillance and search for public health control measures.
Background: Antibiotic-resistant Escherichia coli can colonize the intestinal tract of healthy children, causing concern when antibiotic resistance is related to the presence of transferable mechanisms, such as extended-spectrum beta-lactamases (ESBLs). Materials and Methods: Fecal samples from 41 healthy children from two villages of rural Peru were cultured on ceftriaxone-disks. ESBL production was confirmed with double disk synergy. In all ESBL-produced isolates, antibiotic susceptibility to 12 antibacterial agents was established by disk diffusion, while clonal relationships were determined by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). Presence of ST131 was determined using PCR. Results: Ceftriaxone-resistant microorganisms were recovered from 39 samples belonging to 22 out of 41 children (53.7%). Of these, 80 ceftriaxone-resistant and two ceftriaxoneintermediate E. coli from inside ceftriaxone-halos were confirmed as ESBL-producers. All isolates were multidrug-resistant. In 79/80 (98.8%) ceftriaxone-resistant isolates, the presence of bla(CTX-M) was detected alone (58 isolates, or together with other beta-lactamase (bla(TEM), 17 isolates; bla(OXA-1-like), 3 isolates; bla(TEM) + bla(OXA-1-like),1 isolate), while in one isolate no such ESBL was identified. The two ceftriaxone-intermediate isolates recovered from the same sample, carried a bla(TEM) and bla(SHV) respectively. Thirty-four different clones were identified, with 4 clones being recovered from different samples from the same child. Twelve clones were disseminated among different children, including 5 clones disseminated between both villages. Two clones, accounting for 3 isolates and both recovered from the same children, belonged to E. coli ST131. Conclusion: This study demonstrates high prevalence of ESBL-carriers among healthy children living in a rural area of Peru, stressing the need for continuous surveillance and search for public health control measures.

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