期刊
JOURNAL OF THE ROYAL SOCIETY INTERFACE
卷 19, 期 189, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsif.2021.0903
关键词
cancer; melanoma; individual-based model; FUCCI; population dynamics
资金
- Australian Research Council [DP200100177]
- Australian Government
- Australian Research Council [DP200100177] Funding Source: Australian Research Council
In vitro tumour spheroids have been widely used in studying avascular tumour growth and drug design. The recent development of real-time fluorescent cell cycle imaging enables the monitoring of individual cells within the spheroid, leading to the concept of a four-dimensional tumour spheroid.
In vitro tumour spheroids have been used to study avascular tumour growth and drug design for over 50 years. Tumour spheroids exhibit heterogeneity within the growing population that is thought to be related to spatial and temporal differences in nutrient availability. The recent development of real-time fluorescent cell cycle imaging allows us to identify the position and cell cycle status of individual cells within the growing spheroid, giving rise to the notion of a four-dimensional (4D) tumour spheroid. We develop the first stochastic individual-based model (IBM) of a 4D tumour spheroid and show that IBM simulation data compares well with experimental data using a primary human melanoma cell line. The IBM provides quantitative information about nutrient availability within the spheroid, which is important because it is difficult to measure these data experimentally.
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