期刊
JOURNAL OF MICROBIOLOGY
卷 60, 期 5, 页码 461-468出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-022-1640-z
关键词
Klebsiella; crrAB; kexD; colistin resistance
类别
资金
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Korean government (MSIT) [NRF-2018M3A9H4055197, NRF-2022R1A2B5B02001716]
- Basic Science Research Program of the National Research Foundation (NRF) - Korean government (MSIT) [NRF-2018M3A9H4055197, NRF-2022R1A2B5B02001716]
- Korea Medical Institute (KMI)
- National Research Foundation of Korea [2018M3A9H4055197] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The CrrAB two-component regulatory system is associated with colistin resistance in Klebsiella pneumoniae. This study found significant structural variations in the crrBAC-kexD cluster and its surrounding regions in K. pneumoniae genomes. The cluster may have been acquired from other bacterial species by the ancestor of the K. pneumoniae complex and lost and re-acquired in K. pneumoniae strains. The dynamic evolution of the cluster suggests that it may have additional roles in bacterial physiology, in addition to colistin resistance.
CrrAB two-component regulatory system is associated with colistin resistance in Klebsiella pneumoniae. Recently, some K. pneumoniae isolates lacking crrAB genes have been identified. In this study, we investigated the distribution and structural variation of the crrBAC-kexD cluster. To evaluate the structural variation of the crrBAC-kexD cluster, we explored 59 clinical K. pneumoniae isolates from Korea, and 508 whole genomes of K. pneumoniae and other strains of Klebsiella sp. Significant structural variations in crrBAC-kexD and its surrounding regions were identified among K. pneumoniae genomes. Within the genus Klebsiella, the cluster was identified only in K. pneumoniae, K. variicola, and K. quasipneumoniae, which form the K. pneumoniae complex. Among the 304 available K. pneumoniae genomes, an intact crrBAC-kexD cluster was identified in 178 isolates (58.6%), while the cluster was absent in 90 isolates (29.6%). Partial deletions within the cluster were identified in 22 genomes (7.2%). The most diverse structural patterns of the crrBAC-kexD cluster were observed in ST11 strains. Some clades lacked the crrBAC-kexD cluster. The crrBAC-kexD cluster was identified in the genomes of other bacterial species, including Citrobacter freundii and Enterobacter ludwigii. The crrBAC-kexD cluster is proposed to have been acquired by the ancestor of the K. pneumoniae complex from other bacterial species and the cluster may have been lost and re-acquired repeatedly in K. pneumoniae strains according to the phylogenetic analysis. The dynamic evolution of the crrBAC-kexD cluster suggests that it may have other roles, in addition to colistin resistance, in bacterial physiology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据