Growth and Microstructural Features in Otoliths of Larval and Juvenile Sinogastromyzon wui (F. Balitoridae, River Loaches) of the Upper Pearl River, China

Otolith growth and microstructural features of fish are essential to the understanding of the early fish lifecycle. This paper assesses the features of otoliths from laboratory-reared larval and juvenile Sinogastromyzon wui (S. wui, 0 to 25 days post-hatching) that were obtained as eggs from the Shilong Reach of Xijiang River between April and August 2021. We observed the development of the three pairs of otoliths (lapilli, sagittae, and asterisci) and compared the shape changes and growth of the lapilli and sagittae, as well as the timing and deposition rate of increments of the lapilli. The lapilli and the sagittae were visible on hatching, whereas the asterisci were present at four days post-hatching (dph). The shape of the sagitta changed more obviously than that of the lapillus, and a strong correlation was observed between sagitta shape changes and fish ontogenesis. The otolith shape greatly modulated during the post-flexion larval stage (Post-FLS), it corresponded with the formation period of individual fins. Analysis of the microstructural features indicated that lapilli were the optimal otolith for age determination and increment deposition rate confirmation. Using regression analysis of the known age and the number of lapillus daily increments, we demonstrated that the lapillus developmental increments were deposited daily, and the first increment formed at two days post-hatching. Our conclusions support employing the lapillus increment deposition rate and the time of the first daily increments in the determination of the age of wild larval and juvenile S. wui.

Automated summary

This study assesses the otolith growth and microstructural features of Sinogastromyzon wui larvae and juveniles. It finds that the lapilli otolith is the most suitable for age determination and increment deposition rate confirmation. The shape changes of the sagittae otolith are closely related to fish ontogenesis. The findings support the use of lapillus increment deposition rate and the timing of the first daily increments for age determination of wild larval and juvenile S. wui.