期刊
NEUROPHOTONICS
卷 3, 期 3, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.NPh.3.3.035001
关键词
photoacoustic-computed tomography; brain structural imaging; deep brain imaging; label free; spectral imaging
资金
- NCI NIH HHS [R01 CA186567] Funding Source: Medline
- NIBIB NIH HHS [R01 EB016963, DP1 EB016986] Funding Source: Medline
- NINDS NIH HHS [U01 NS090579] Funding Source: Medline
Capitalizing on endogenous hemoglobin contrast, photoacoustic-computed tomography (PACT), a deep-tissue high-resolution imaging modality, has drawn increasing interest in neuroimaging. However, most existing studies are limited to functional imaging on the cortical surface and the deep brain structural imaging capability of PACT has never been demonstrated. Here, we explicitly studied the limiting factors of deep brain PACT imaging. We found that the skull distorted the acoustic signal and blood suppressed the structural contrast from other chromophores. When the two effects are mitigated, PACT can potentially provide high-resolution label-free imaging of structures in the entire mouse brain. With 100-mu m in-plane resolution, we can clearly identify major structures of the brain, which complements magnetic resonance microscopy for imaging small-animal brain structures. Spectral PACT studies indicate that structural contrasts mainly originate from cytochrome distribution and that the presence of lipid sharpens the image contrast; brain histology results provide further validation. The feasibility of imaging the structure of the brain in vivo is also discussed. Our results demonstrate that PACT is a promising modality for both structural and functional brain imaging. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
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