期刊
NEW JOURNAL OF CHEMISTRY
卷 46, 期 19, 页码 9331-9343出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2nj00896c
关键词
-
资金
- National Natural Science Foundation of China [21762008, 21562007]
- Natural Science Foundation of Guangxi Province [2019JJA120066]
- BAGUI Scholar Program of Guangxi Province of China
The interaction mechanism between 3-biotinylate-6-benzimidazole B-nor-cholesterol analogs and ct-DNA was studied using spectroscopic, viscosity, and molecular docking methods. The compounds were found to bind to ct-DNA through the groove mode, causing color enhancement and static fluorescence quenching.
The interaction mechanism between 3-biotinylate-6-benzimidazole B-nor-cholesterol analogs and ct-DNA was studied by spectroscopic (ultraviolet spectroscopy (UV), fluorescence spectroscopy, and circular dichroism spectroscopy (CD)), viscosity, and molecular docking technology methods. The UV spectra showed that these compounds induced a color enhancement effect on ct-DNA. The fluorescence spectra verified that the compounds and ct-DNA were bound in the grooves, and the fluorescence quenching process was static quenching. The CD spectra revealed that these compounds did not change the positive peak of the ct-DNA base pair accumulation, while the negative peak of the ct-DNA right-hand helix exhibited a slight hyperchromic effect. The viscosity test indicated that the reaction between these compounds and ct-DNA did not significantly change the viscosity. According to the molecular docking results, the three compounds mainly interacted with the ct-DNA via the groove mode, and all of them expressed a weak electrostatic effect, which was slightly stronger for compound 3.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据