期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 3, 期 7, 页码 1322-1331出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00331
关键词
chondroitin sulfate; hyaluronic acid; fiber-based techniques; microfludics; polyelectrolyte complexation; tendon
资金
- Portuguese funds through FCT Fundacao pars a Ciencia e a Tecnologia of FCT-POPH-FSE [SFRH/BD/96593/2013, SFRH/BPD/103604/2014]
- ON.2 under the National Strategic Reference Framework (NSRF) through the European Regional Development Fund (ERDF) [RL3-TECT-NORTE-07-0124-FEDER-000020]
- European Research Council [ERC-2012-ADG 20120216-321266]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/96593/2013] Funding Source: FCT
Fiber-based techniques hold great potential toward the development of structures that mimic the architecture of fibrous tissues, such as tendon. Microfluidics and polyelectrolyte complexation are among the most widely used techniques for the fabrication of fibrous structures. In this work, we combined both techniques to generate hydrogel fibers with a fibrillar-like structure. For this, either methacrylated hyaluronic acid (MA-HA) or chondroitin sulfate (MA-CS) were mixed with alginate (ALG), being all negatively charged polysaccharides, combined with chitosan (CHT), which is positively charged, and separately injected into a microfluidic device. Through a continuous injection into a coagulation bath and subsequent photo-cross-linking, we could obtain multi component hydrogel fibers, which exhibited smaller fibrils aligned in parallel, whenever CHT was present. The biological performance was assessed upon encapsulation and further culture of tendon cells. Overall, the reported process did not affect cell viability and cells were also able to maintain their main function of producing extracellular matrix up to 21 days in culture. In summary, we developed a novel class of photo-cross-linkable multicomponent hydrogel fibers than can act as bioactive modulators of cell behavior.
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