期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 2, 期 9, 页码 1619-1629出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00375
关键词
bioreactors; cardiovascular; tissue engineering; stretch conditioning; magnetic nanoparticles
资金
- NIH [R01-EB011516]
- Robert A. Welch Foundation [E-1320]
- Department of Biomedical Engineering, the Cullen College of Engineering Texas Center for Superconductivity at the University of Houston
Bioreactor systems, an integral component of tissue engineering, are designed to simulate complex in vivo conditions to impart functionality to artificial tissue. All standard forms of stretch bioreactors require physical contact with artificial heart muscle (AHM). However, we believe that noncontact stretch bioreactors have the potential to lead to higher functional benefit of AHM. Our work is focused on the fabrication of a noncontact magnetic stretch bioreactor (MSB) that uses magnetic nanoparticles to simulate stretch conditions to impart functionality. During our development of this system, we applied magnetically induced stretch conditioning through application of an oscillating magnetic field to a ferromagnetic heart muscle model. Fibrin scaffolds were loaded with magnetic nanoparticles prior to tissue model formation. Oscillating magnetic fields were applied by a novel bioreactor system through displacement of a neodymium magnet. The addition of commercially obtained iron(III) oxide (Fe2O3) in sufficient quantities to allow for physiologically relevant stretches (15% axial displacement) caused toxic effects after 4 days of culture. In contrast, loading scaffolds with monodispersed, high-saturation-magnetization magnetite (Fe3O4) nanoparticles specifically prepared for these experiments increased the field strength of the magnetized fibrin 10-fold over polydispersed, low-saturation magnetization, Fe2O3. Additionally, loading with Fe3O4 enabled magnetically actuated stretching with markedly reduced toxicity over 8 days of culture. Using a 20% stretch 0.5 Hz protocol, we observed a significant increase in twitch force over controls at days 4 and 6. This work provides a technology for controlled noncontact mechanical stretch to condition AHM.
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