期刊
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
卷 10, 期 3, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jece.2022.107488
关键词
SARS-CoV-2; RT-LAMP; Electrochemical sensor; Redox intercalator; Wastewater; Epidemiology
资金
- DGAPA-UNAM-PAPIIT [TA100221]
The study introduces a simple, low-cost, and affordable electrochemical sensor for specific detection of the genes of the novel coronavirus. Testing with wastewater samples demonstrates the sensor's performance and reliability.
The current pandemic COVID-19 caused by the coronavirus SARS-CoV-2, has generated different economic, social and public health problems. Moreover, wastewater-based epidemiology could be a predictor of the virus rate of spread to alert on new outbreaks. To assist in epidemiological surveillance, this work introduces a simple, low-cost and affordable electrochemical sensor to specifically detect N and ORF1ab genes of the SARS-CoV-2 genome. The proposed sensor works based on screen-printed electrodes acting as a disposable test strip, where the reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction takes place. Electrochemical detection relies upon methylene blue as a redox intercalator probe, to provide a diffusion-controlled current encoding the presence and concentration of RT-LAMP products, namely amplicons or double-stranded DNA. We test the performance of the sensor by testing real wastewater samples using end-point and time course measurements. Results show the ability of the electrochemical test strip to specifically detect and quantify RT-LAMP amplicons below to ~ 2.5 x 10(-6) ng/mu L exhibiting high reproducibility. In this sense, our RT-LAMP electrochemical sensor is an attractive, efficient and powerful tool for rapid and reliable wastewater-based epidemiology studies.
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