期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 2, 期 4, 页码 634-642出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00035
关键词
zinc; stents; in vitro testing; cytotoxicity; biocompatibility
资金
- U.S. National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering [5R21 EB 019118-02]
- National Heart, Lung, and Blood Institute [1R15HL115521-01A1, 1R15HL129199-01]
- Michigan Technological University
- American Heart Association
Zinc (Zn) and its alloys have recently been introduced as a new class of biodegradable metals with potential application in biodegradable vascular stents. Although an in vivo feasibility study pointed to outstanding biocompatibility of Zn-based implants in vascular environments, a thorough understanding of how Zn and Zn2+ affect surrounding cells is lacking. In this comparative study, three vascular cell types human endothelial cells (HAEC), human aortic smooth muscle cells (AoSMC), and human dermal fibroblasts (hDF) were studied to advance the understanding of Zn/Zn2+-cell interactions. Aqueous cytotoxicity using a Zn2+ insult assay resulted in LD50 values of 50 mu M for hDF, 70 mu M for AoSMC, and 265 mu M for HAEC. Direct cell contact with the metallic Zn surface resulted initially in cell attachment, but was quickly followed by cell death. After modification surface using a layer of gelatin intended to mimic a protein layer seen in vivo the cells were able to attach and proliferate on the Zn surface. Further experiments demonstrated a Zn dose-dependent effect on cell spreading and migration, suggesting that both adhesion and cell mobility may be hindered by free Zn2+.
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