期刊
FOOD CHEMISTRY: MOLECULAR SCIENCES
卷 4, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fochms.2022.100086
关键词
Crocetin esters; Crocus sativus; Flow cytometry; Picrocrocin; Relative gene expression; Safranal
资金
- Campus of Agriculture and Natural Resources, University of Tehran, Iran
The study investigated the effects of colchicine and ethyl methanesulfonate (EMS) mutagens on inducing new variations in Crocus sativus L. The results suggest the potential of mutagens in creating new variations in the plant.
Crocus sativus L., also known as saffron, is one of the most important medicinal and spice plants throughout the world. The plant is a rich source of apocamtenoids such as crocetin esters, picrocrocin, and safranal. The purpose of this study was to investigate the effect of colchicine and ethyl methanesulfonate (EMS) mutagens on possible inducing new variation in C. sativus. Accordingly, corms were exposed to EMS (0.1% and 0.2%) and colchicine (0.05% and 0.025%) for three incubation times. The lowest survival rate of corms was related to EMS treatments. The relative expression of ALDH, BGL, and CCD2 genes under 0.025% colchicine treatment for 12 h revealed a 2 fold increase compared with the control. The flow cytometric measurements (FCM) of the nuclear DNA content of the colchicine-treated plants did not reveal any significant changes in 2C DNA content. The results, manifest the potential of mutagens to create new variations in the plant.
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