期刊
MOLECULAR SYSTEMS DESIGN & ENGINEERING
卷 7, 期 8, 页码 906-914出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2me00014h
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资金
- Mississippi INBRE [P20GM103476]
- National Science Foundation [CBET MRI 2019023, OIA 1757220]
In this work, a donor-acceptor-donor fluorophore was encapsulated using a linear dendritic block copolymer, and a polyethylene glycol derivative was synthesized. The self-assembly, colloidal properties, photophysical and morphological characteristics of the nanoaggregates were compared. Both types of nanoaggregates showed low cytotoxicity and accumulated in the lysosomes of HEK-293 cells. These findings provide interesting insights into NIR fluorophore design and methods to modify the properties of nanoaggregates for bio-imaging.
In this work, we have taken a donor-acceptor-donor (D-A-D) fluorophore (II-EDOT-TPA) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative (PEG-II-EDOT-TPA) was synthesized. The self-assembly and colloidal properties of both nanoaggregates were comparatively assessed. Photophysical and morphological characterization of the LDBC encapsulated II-EDOT-TPA and PEG-II-EDOT-TPA nanoaggregates was performed, which showed the photophysical and morphological properties differed greatly when comparing the two. Both nanoaggregate types were incubated with HEK-293 cells in order to measure cell viability and perform confocal fluorescence microscopy. Minimal cytotoxicity values (<20%) were seen with the two nanoaggregate forms, while both types of nanoaggregates were found to accumulate into the lysosomes of the HEK-293 cells. This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.
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