Highly catalysis amplification of MOFNd-loaded nanogold combined with specific aptamer SERS/RRS assay of trace glyphosate

A neodymium metal-organic framework (MOFNd) was prepared using 1H-pyrazole-3,5-dicarboxylic acid (H(3)pdc) and 2-pyrazinecarboxylic acid as ligands. Through the addition of HAuCl4 as a precursor and NaBH4 as a reducing agent, a new MOFNd-loaded nanogold (AuNPs) (Au@MOFNd) nanosol with good stability and high catalytic activity was conveniently prepared via a solvothermal-reduction method and characterized. It was found that the indicator reaction of reducing HAuCl4 by Na2SO3 to generate AuNPs was slow. Au@MOFNd strongly catalyzes this nanoreaction, and the produced AuNPs exhibit a strong resonance Rayleigh scattering (RRS) peak at 370 nm, and a strong surface-enhanced Raman scattering (SERS) peak at 1617 cm(-1) with the addition of the molecular probe Victoria blue 4R (VB4r). A novel SERS/RRS di-mode quantitative analysis method for glyphosate (GLY) was established by coupling this new Au@MOFNd catalytic indicator reaction with the aptamer (Apt) reaction of GLY, with SERS and RRS detection limits of 0.02 nM and 0.3 nM, respectively. It has been applied to the analysis of soil samples with a recovery rate of 93.0%-106.5% and precision of 2.2%-4.1%, and the results were satisfactory.

Automated summary

A highly catalytic Au@MOFNd nanosol was prepared using a solvothermal-reduction method, and a novel SERS/RRS di-mode quantitative analysis method for glyphosate was established.