4.4 Review

Developments and perspectives in high-throughput protein glycomics: enabling the analysis of thousands of samples

期刊

GLYCOBIOLOGY
卷 32, 期 8, 页码 651-663

出版社

OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwac026

关键词

glycomics; glycoproteomics; high-throughput; mass spectrometry; population studies

资金

  1. European Structural and Investment Funds IRI CardioMetabolic grant [KK.01.2.1.02.0321]
  2. Centre of Competence in Molecular Diagnostics [KK.01.2.2.03.0006]
  3. Croatian National Centre of Research Excellence in Personalized Healthcare [KK.01.1.1.01.0010]
  4. Dutch Research Council project Proteoform-resolved pharmacokinetics of biopharmaceuticals [ENPPS.LIFT.019.012]
  5. European Research Council (ERC) [772735]
  6. European Research Council (ERC) [772735] Funding Source: European Research Council (ERC)

向作者/读者索取更多资源

Glycans enhance the structural complexity of proteins, and glycosylation is an important regulatory mechanism in many biological processes. Due to extensive interindividual differences in glycosylation, large studies are needed to map the structures and understand the role of glycosylation in human physiology. High-throughput glycomics methods have emerged to tackle the complexity of glycosylation and extensively characterize the human glycome in various biological samples. The ultimate goal is to gain better knowledge of the complete human glycome using easily implementable methods in biomedical research.
Glycans expand the structural complexity of proteins by several orders of magnitude, resulting in a tremendous analytical challenge when including them in biomedical research. Recent glycobiological research is painting a picture in which glycans represent a crucial structural and functional component of the majority of proteins, with alternative glycosylation of proteins and lipids being an important regulatory mechanism in many biological and pathological processes. Since interindividual differences in glycosylation are extensive, large studies are needed to map the structures and to understand the role of glycosylation in human (patho)physiology. Driven by these challenges, methods have emerged, which can tackle the complexity of glycosylation in thousands of samples, also known as high-throughput (HT) glycomics. For facile dissemination and implementation of HT glycomics technology, the sample preparation, analysis, as well as data mining, need to be stable over a long period of time (months/years), amenable to automation, and available to non-specialized laboratories. Current HT glycomics methods mainly focus on protein N-glycosylation and allow to extensively characterize this subset of the human glycome in large numbers of various biological samples. The ultimate goal in HT glycomics is to gain better knowledge and understanding of the complete human glycome using methods that are easy to adapt and implement in (basic) biomedical research. Aiming to promote wider use and development of HT glycomics, here, we present currently available, emerging, and prospective methods and some of their applications, revealing a largely unexplored molecular layer of the complexity of life.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据