期刊
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
卷 10, 期 3, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jece.2022.107478
关键词
Wastewater-based epidemiology; SARS-CoV-2; Environmental surveillance; Sampling techniques; Sewage
资金
- Brazilian National Council of Scientific and Technological Development (CNPq)
- Ministry of Science, Technology, Innovations and Communications (MCTIC)
- Ministry of Health (MS), Secretariat of Science, Technology, Innovation and Strategic Inputs [Decit/SCTIE 07/2020, 402432/2020-7]
This study evaluated the variability of SARS-CoV-2 RNA concentrations in wastewater samples. The results showed good agreement between different sampling methods and low variability in viral concentrations throughout the day. Therefore, this study provides important information on wastewater sampling techniques for COVID-19 surveillance.
The shedding of SARS-CoV-2 RNA titers by infected individuals, even asymptomatic and oligosymptomatic ones, allows the use of wastewater monitoring to track the COVID-19 spread in a community. This approach is interesting especially for emerging countries with limited clinical testing capabilities. However, there are still important methodological aspects that need validation so that wastewater monitoring data become more representative and useful for public health. This study evaluated the between-day and within-day variability of SARS-CoV-2 RNA concentrations in 24-hour composite and grab samples from three different sampling points, including two wastewater treatment plants (WTTP) and a sewer manhole. In the between-day evaluation (17 weeks of monitoring), a good agreement between the SARS-CoV-2 RNA concentration of each sampling method was observed. There were no significant differences between the mean concentrations of the grab and composite samples (p-value > 0.05), considering N1 and N2 gene assays. The strong relationship between composite and grab samples was proven by correlation coefficients: Pearson's r of 0.83 and Spearman's rho of 0.78 (p-value < 0.05). In within-day evaluation, 24-hour cycles were analyzed and low variability in hourly viral concentrations was observed for three sampling points. The coefficient of variation (CV) values ranged from 3.0% to 11.5%. Overall, 24-hour profiles showed that viral RNA concentrations had less variability and greater agreement with the mean values between 8 a.m. and 10 a.m, the recommended time for grab sampling. Therefore, this study provides important information on wastewater sampling techniques for COVID-19 surveillance. Wastewater monitoring information will only be useful to public health and decision-makers if we ensure data quality through best practices.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据