Bacteroides ovatus-mediated CD27- MAIT cell activation is associated with obesity-related T2D progression

Type 2 diabetes (T2D) is highly associated with obesity. However, the factors that drive the transition from excessive weight gain to glucose metabolism disruption are still uncertain and seem to revolve around systemic immune disorder. Mucosal-associated invariant T (MAIT) cells, which are innate-like T cells that recognize bacterial metabolites, have been reported to be altered in obese people and to lead to metabolic dysfunction during obesity. By studying the immunophenotypes of blood MAIT cells from a cross-sectional cohort of obese participants with/without T2D, we found an elevation in CD27-negative (CD27(-)) MAIT cells producing a high level of IL-17 under T2D obese conditions, which could be positively correlated with impaired glucose metabolism in obese people. We further explored microbial translocation caused by gut barrier dysfunction in obese people as a triggering factor of MAIT cell abnormalities. Specifically, accumulation of the bacterial strain Bacteroides ovatus in the peripheral blood drove IL-17-producing CD27(-) MAIT cell expansion and could be associated with T2D risk in obese individuals. Overall, these results suggest that an aberrant gut microbiota-immune axis in obese people may drive or exacerbate T2D. Importantly, CD27(-) MAIT cell subsets and Bacteroides ovatus could represent targets for novel interventional strategies. Our findings extend current knowledge regarding the clinical relevance of body mass index (BMI)-associated variation in circulating MAIT cells to reveal the role of these cells in obesity-related T2D progression and the underlying cellular mechanisms.

Automated summary

Type 2 diabetes (T2D) is closely associated with obesity, and the factors driving the transition from excessive weight gain to glucose metabolism disruption are still uncertain. This study found that CD27(-) MAIT cells in the blood of obese individuals with T2D were elevated and produced high levels of IL-17, which may be correlated with impaired glucose metabolism in obese people. Additionally, gut barrier dysfunction and the accumulation of Bacteroides ovatus bacteria in the peripheral blood were found to contribute to the abnormality of MAIT cells and may be associated with T2D risk in obese individuals.