期刊
CURRENT RESEARCH IN FOOD SCIENCE
卷 5, 期 -, 页码 196-206出版社
ELSEVIER
DOI: 10.1016/j.crfs.2021.12.015
关键词
Browning reactions; Tyrosinase; Heterologous expression; Enzyme characterization; Individual phenolics; Antioxidants
资金
- Austrian Science Fund (FWF) [P32326]
- Algerian Ministry of Higher Education and Scientific Research
- Algerian Ministry of Higher Education and Scientific Research (MESRS)
- Austrian Science Fund (FWF) [P32326] Funding Source: Austrian Science Fund (FWF)
In this study, the effect of enzymatic browning on the phenolic composition of apricot was investigated. The results showed that browning significantly decreased total phenolics and antioxidant capacity, with catechin, epicatechin, and B-type procyanidins being the most affected phenolics.
In this study, we investigate the effect of enzymatic browning on the phenolic composition of apricot in vivo and in vitro. The in vitro browning was caused by the recombinant latent apricot polyphenol oxidase (L-PaPPO). Successful heterologous expression of PaPPO in Escherichia coli yielded substantial amounts of enzyme containing both copper ions in the catalytic active site. The expressed L-PaPPO was characterized with regard to its molecular mass (56531.3 Da), pH optimum (7.0), activation by SDS, and enzyme kinetics. LC-MS/MS was used to compare the phenolic profiles of brown and non-brown apricots. The browning reactions did significantly decrease total phenolics and antioxidant capacity (measured with DPPH and CUPRAC assays). Catechin, epicatechin, and B-type procyanidins were the individual phenolics most affected by browning, followed by chlorogenic and neochlorogenic acid. These phenolics are most likely the main endogenous substrates of LPaPPO, as they were oxidized much faster than the other identified phenolics.
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