期刊
FRONTIERS IN CHEMISTRY
卷 4, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2016.00042
关键词
Chagas disease; trypanosome; bloodstream trypomastigote; secretome; glycoprotein; phosphoprotein
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- FAPEG
- FAPDF (Fundacao de Amparo a Pesquisa do Estado de Goias e Distrito Federal) [563998/ 2010-5]
- CAPES Programa Nacional de Incentivo a Pesquisa em Parasitologia Basica (CAPES) [23038.005298/2011-83]
- DPP/UnB
- FINEP (Financiadora de Estudos e Projetos), programme CAPES-COFECUB [723/11]
- Villum Fonden [00007292] Funding Source: researchfish
The protozoan parasite Trypanosoma cruzi causes Chagas disease, one of the major neglected infectious diseases. It has the potential to infect any nucleated mammalian cell. The secreted/excreted protein repertoire released by T. cruzi trypomastigotes is crucial in host pathogen interactions. In this study, mammalian tissue culture-derived trypomastigotes (Y strain) were used to characterize the exoproteome of the infective bloodstream life form. Proteins released into the serum-free culture medium after 3 h of incubation were harvested and digested with trypsin. NanoLC-MS/MS analysis resulted in the identification of 540 proteins, the largest set of released proteins identified to date in Trypanosoma spp. Bioinformatic analysis predicted most identified proteins as secreted, predominantly by non-classical pathways, and involved in host-cell infection. Some proteins possess predicted GPI-anchor signals, these being mostly trans-sialidases, mucin associated surface proteins and surface glycoproteins. Moreover, we enriched phosphopeptides and glycopeptides from tryptic digests. The majority of identified glycoproteins are trans-sialidases and surface glycoproteins involved in host-parasite interaction. Conversely, most identified phosphoproteins have no Gene Ontology classification. The existence of various proteins related to similar functions in the exoproteome likely reflects this parasite's enhanced mechanisms for adhesion, invasion, and internalization of different host-cell types, and escape from immune defenses.
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