2-Methoxy-1,4-naphthoquinone regulated molecular alternation of Fusarium proliferatum revealed by high-dimensional biological data

Fungi Fusarium proliferatum and the toxins it produces are hazardous to agricultural plants, animals, and human health. However, there is a lack of more effective and environment-friendly natural anti-F. proliferatum agents. In the search for natural anti-fungal agents, we found that naturally originated 2-methoxy-1,4-naphthoquinone (MNQ) with a minimal inhibitory dose of 8.0 mg L-1 possessed a potential inhibitory effect on F. proliferatum. The results of transcriptomic, proteomic, and metabolomic reveal a total of 1314 differential expression genes (DEGs, 873 up-regulated and 441 down-regulated), 259 differential expression proteins (DEPs, 104 up-regulated and 155 down-regulated), and 86 differential accumulation metabolites (DAMs, 49 up-regulated and 37 down-regulated) in MNQ-induced F. proliferatum. Further, the correlation analysis of transcriptomic, proteomic, and metabolomic indicated that these DEGs, DEPs, and DAMs were co-mapped in the pathways of glyoxylate and dicarboxylate metabolism, glycine, serine, and threonine metabolism, and pyruvate metabolism that linked to the TCA cycle. Furthermore, the key DEGs of the significantly co-mapped pathways were verified with qPCR analysis, which was related to the permeability of the cell membrane of F. proliferatum. Thus, these findings will provide fundamental scientific data on the molecular shifts of MNQ-induced F. proliferatum.

Automated summary

Fungi Fusarium proliferatum and its toxins are harmful to agriculture, animals, and human health. This study discovered that naturally derived 2-methoxy-1,4-naphthoquinone (MNQ) has potential inhibitory effects on F. proliferatum and explored the molecular mechanisms involved.