期刊
NANOTECHNOLOGY REVIEWS
卷 11, 期 1, 页码 1911-1926出版社
DE GRUYTER POLAND SP Z O O
DOI: 10.1515/ntrev-2022-0114
关键词
AgNPs; HT-29; cytotoxicity
类别
资金
- National Natural Science Foundation of China [82072704, 81973525]
- Jiangsu Primary Research & Development Plan [SBE2021740280]
- 333 Talents Program of Jiangsu Province [BRA2020390]
- Project of National Clinical Research Base of Traditional Chinese Medicine in Jiangsu Province [JD2022SZXYA01]
This study synthesized AgNPs using gallic acid as a reductant and characterized them using various techniques. The synthesized AgNPs exhibited cytotoxicity on colorectal adenocarcinoma cells (HT-29) and induced apoptosis and non-canonical autophagy. Further research using animal models is needed to understand the exact mechanisms of AgNPs in the treatment of digestive tract neoplasms.
The interactions of nanomaterials with biological materials such as immortalized cell lines are recently on the rise. Owing to this superiority, the biosynthesis of AgNPs using gallic acid as a reductant was implemented in this study. After being synthesized, the AgNPs were characterized using techniques such as dynamic light scattering, transmission electron microscopy, selected area electron diffraction, and X-ray diffraction methods. Furthermore, the AgNPs were assessed for their cytotoxic effects on the colorectal adenocarcinoma cell line HT-29. The mechanisms of such cell-killing effect were investigated by analyzing the expressions of 14 mRNAs using quantitative polymerase chain reaction. The outcomes indicate that the synthesized AgNPs were cytotoxic on HT-29 cells. The expressions of all apoptotic genes analyzed including cyt-C, p53, Bax, Bcl2, CASP3, CASP8, CASP9, and CASP12 were upregulated. With regard to the autophagy-related genes, Beclin-1, XBP-1, CHOP, and LC3-II were upregulated, whereas the expressions of ATG3 and ATG12 were downregulated. To conclude, the AgNPs induced mitochondria-dependent apoptosis and non-canonical autophagy in HT-29 cells. A crosstalk did occur between autophagy and apoptosis in such a cell-killing effect. Hence, further studies are required to elucidate the exact mechanisms in animal models for further use of AgNPs in clinical medicine for the treatment of neoplasms of the digestive tract.
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