期刊
BIOPHYSICS REVIEWS
卷 3, 期 2, 页码 -出版社
AIP Publishing
DOI: 10.1063/5.0082799
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资金
- S.P. thanks the University of Lausanne and the Swiss National Science Foundation for support. K.M.S. acknowledges support by the Human Frontier Science Program (career development award), the German Research Foundation (DFG Project No. 431480687), and the
- University of Lausanne
- Swiss National Science Foundation
- Human Frontier Science Program [431480687]
- German Research Foundation
- Helmholtz Gesellschaft [391105827]
- German Research Foundation (DFG)
This article summarizes the key points to consider when setting up and analyzing a live-cell imaging experiment, with a special focus on yeast. It also discusses the strategies for improving reproducibility in the field through reporting and data sharing.
Live-cell microscopy is a powerful tool that can reveal cellular behavior as well as the underlying molecular processes. A key advantage of microscopy is that by visualizing biological processes, it can provide direct insights. Nevertheless, live-cell imaging can be technically challenging and prone to artifacts. For a successful experiment, many careful decisions are required at all steps from hardware selection to downstream image analysis. Facing these questions can be particularly intimidating due to the requirement for expertise in multiple disciplines, ranging from optics, biophysics, and programming to cell biology. In this review, we aim to summarize the key points that need to be considered when setting up and analyzing a live-cell imaging experiment. While we put a particular focus on yeast, many of the concepts discussed are applicable also to other organisms. In addition, we discuss reporting and data sharing strategies that we think are critical to improve reproducibility in the field.
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