AI and computational chemistry-accelerated development of an alotaketal analogue with conventional PKC selectivity

The protein kinase C (PKC) family consists of ten isozymes and is a potential target for treating cancer, Alzheimer's disease, and HIV infection. Since known natural PKC agonists have little selectivity among the PKC isozymes, a new scaffold is needed to develop PKC ligands with remarkable isozyme selectivity. Taking advantage of machine-learning and computational chemistry approaches, we screened the PubChem database to select sesterterpenoids alotaketals as potential PKC ligands, then designed and synthesized alotaketal analogues with a different ring system and stereochemistry from the natural products. The analogue exhibited a one-order higher affinity for PKC alpha-C1A than for the PKC delta-C1B domain. Thus, this compound is expected to serve as the basis for developing PKC ligands with isozyme selectivity.

Automated summary

Protein kinase C (PKC) family is a potential target for treating cancer, Alzheimer's disease, and HIV infection. By screening compounds and designing analogues, we discovered a PKC ligand with remarkable isozyme selectivity.