期刊
CRYOLETTERS
卷 43, 期 1, 页码 50-57出版社
CRYO LETTERS
DOI: 10.54680/fr22110110612
关键词
alpha tocopherol; drug release; liposome; sperm cryopreservation; stability
资金
- 'Associated Laboratory in Marine Ecosystems and Aquaculture, Faculty of Nature and Life Sciences' University of Bejaia
- 'Technology Pharmaceutical Laboratory, Department of Processes Engineering, Faculty of Technology' University of Bejaia
- Bio pharmaceutics Laboratory, UFR Medicine and Pharmacy, Rouen University
This study successfully encapsulated alpha tocopherol into liposomes and demonstrated its stability and drug release effect in sperm cryopreservation. The use of vitamin E liposome preparations significantly improved sperm motility compared to other treatments, indicating potential applications.
BACKGROUND: It is known that a considerable number of drugs in clinical use or under development are water-insoluble drugs with poor bioavailability. The liposomal delivery system has drawn attention as one of the noteworthy approaches to increase both dissolution and absorption because of its biocompatibility and ability to encapsulate hydrophobic molecules in the lipid domain. However, several drawbacks have been reported, the most common is liposome structural instability. OBJECTIVE: To encapsulate alpha tocopherol into liposomes, to determine the new formulation stability and to study the drug-release of alpha tocopherol into the sperm cryopreservation medium. MATERIALS AND METHODS: The liposomes prepared by an ethanol injection method were characterized for size stability, alpha tocopherol release and sperm motility tests. RESULTS: The prepared unilamellar vesicles had both narrow size distribution (around 99 nm) and a good physical and chemical stability at 4 degrees C during 12 months. The liposomes did not release the vitamin E immediately, but retained the protectant for 24 hours, probably due to the rigidity of the liposomal fence which was reinforced by adding cholesterol. Then, all vitamin E molecules were released by 48 hours. Release was potentially by Fickian diffusion probably by the creation of mini-ducts due to both agitation and fence hydration. Moreover, semen motility treated with vitamin E liposome preparations was significantly improved compared to all other treatments (including commonly used sperm conservation media). CONCLUSION: The stable vitamin E liposomes formulated in this work are a promising alternative for semen cryopreservation protection.
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