期刊
CHEMICAL COMMUNICATIONS
卷 58, 期 48, 页码 6857-6860出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2cc02085h
关键词
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资金
- Darwin Studentship - EPSRC [EP/S002979/1]
- BBSRC [BB/S019456/1]
- BBSRC [BB/S019456/1] Funding Source: UKRI
In this study, we demonstrated that intact non-covalent protein complexes can be directly detected from bacterial colonies growing on agar by combining electroporation with native ambient mass spectrometry. Homodimers HdeA and HdeB, as well as the 50 kDa Mn-bound superoxide dismutase homodimer, were identified, along with some previously undetected monomeric proteins.
Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.
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