期刊
NANOSCALE
卷 14, 期 21, 页码 7934-7942出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1nr07362a
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资金
- Singapore University of Technology and Design [SUTDT12017003]
- Changi General Hospital (Singapore) [CGH-SUTD-HTIF2019-001]
- Ministry of Education (Singapore) [MOE-T2EP50220-0022]
- SUTD-Zhejiang University (SUTD-ZJU) [201903]
- Agency of Science Technology and Research (Singapore) [A20G9b0135]
- Massachusetts Institute of Technology-SUTD International Design Centre
- National Supercomputing Centre, Singapore [15001618]
- SUTD President Graduate Scholarship
This study investigates the control of membrane permeabilization of cancer cells using different numbers of electric-field pulses and explores the potential of few-volt nanosecond AC-stimulation with an increased number of pulse strategies for the development of next-generation low-power electroporation systems.
The growing importance of applications based on molecular medicine and genetic engineering is driving the need to develop high-performance electroporation technologies. The electroporation phenomenon involves disruption of the cell for increasing membrane permeability. Although there is a multitude of research focused on exploring new electroporation techniques, the engineering of programming schemes suitable for these electroporation methods remains a challenge. Nanosecond stimulations could be promising candidates for these techniques owing to their ability to generate a wide range of biological responses. Here we control the membrane permeabilization of cancer cells using different numbers of electric-field pulses through orientational disordering effects. We then report our exploration of a few-volt nanosecond alternating-current (AC) stimulation method with an increased number of pulses for developing electroporation systems. A recovery time of similar to 720 min was achieved, which is above the average of similar to 76 min for existing electroporation methods using medium cell populations, as well as a previously unreported increased conductance with an increase in the number of pulses using weak bias amplitudes. All-atom molecular dynamics (MD) simulations reveal the orientation-disordering-facilitated increase in the degree of permeabilization. These findings highlight the potential of few-volt nanosecond AC-stimulation with an increased number of pulse strategies for the development of next-generation low-power electroporation systems.
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