期刊
ACS CENTRAL SCIENCE
卷 2, 期 12, 页码 885-895出版社
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.6b00222
关键词
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资金
- Medical Research Council
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council UK Regenerative Medicine Platform Hubs [MR/K026682/1, MR/K026666/1]
- Medical Engineering Solutions in the Osteoarthritis Centre of Excellence
- Wellcome Trust
- Engineering and Physical Sciences Research Council [088844]
- Value in People Award from the Wellcome Trust Institutional Strategic Support Fund [097816/Z/11/A]
- ERC Seventh Framework Programme Consolidator grant Naturale CG [616417]
- Nano Doctoral Training Centre (NanoDTC)
- University of Cambridge
- EPSRC [EP/G037221/1]
- Wellcome Trust [097816/Z/11/A] Funding Source: Wellcome Trust
- MRC [MR/K026666/1, MR/K026682/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [1208951] Funding Source: researchfish
- Medical Research Council [MR/K026682/1, MR/K026666/1] Funding Source: researchfish
Tissue architecture is intimately linked with its functions, and loss of tissue organization is often associated with pathologies. The intricate depth-dependent extracellular matrix (ECM) arrangement in articular cartilage is critical to its biomechanical functions. In this study, we developed a Raman spectroscopic imaging approach to gain new insight into the depth-dependent arrangement of native and tissue-engineered articular cartilage using bovine tissues and cells. Our results revealed previously unreported tissue complexity into at least six zones above the tidemark based on a principal component analysis and k-means clustering analysis of the distribution and orientation of the main ECM components. Correlation of nanoindentation and Raman spectroscopic data suggested that the biomechanics across the tissue depth are influenced by ECM microstructure rather than composition. Further, Raman spectroscopy together with multivariate analysis revealed changes in the collagen, glycosaminoglycan, and water distributions in tissue-engineered constructs over time. These changes were assessed using simple metrics that promise to instruct efforts toward the regeneration of a broad range of tissues with native zonal complexity and functional performance.
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