4.5 Review

Critical review of antibiotic resistance genes in the atmosphere

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Global dispersal and potential sources of antibiotic resistance genes in atmospheric remote depositions

Joan Caliz et al.

Summary: Antibiotic resistance has become a major global health concern. This study investigates the atmospheric deposition of antibiotic resistance genes (ARGs) in a remote high mountain area and finds that ARGs can be dispersed long-range and persistently through aerosols in the free troposphere.

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Seasonal disparities and source tracking of airborne antibiotic resistance genes in Handan, China

Qing Wang et al.

Summary: This study reveals that the absolute abundance of ARG subtypes in winter is generally twice as high as in summer, possibly due to winter haze events with high particulate matter concentrations in Handan. Exogenous input from serious haze events and local release of ARGs loaded on PM2.5 of air masses may cause higher levels of ARGs in the winter.

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Dong Wu et al.

Summary: The study found that airborne fine particulate matter (PM2.5) emitted from hospitals contains a higher abundance of antibiotic resistance genes (ARGs) compared to urban ambient air PM2.5. The ARGs are closely associated with human-source-influenced bacterial communities, indicating the presence of potential antibiotic-resistant bacteria. The transfer potential of ARGs remains high in hospital air PM samples, and is related to beta-lactam-resistant infections within the hospital. Additionally, the study reveals that the risk of exposure to potentially virulent bacteria through inhalation of PM2.5 is ten times greater than through drinking water ingestion.

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Characteristics of airborne bacterial communities and antibiotic resistance genes under different air quality levels

Xu Yan et al.

Summary: This study investigated the microbial community structure and distribution of antibiotic resistance genes (ARGs) in airborne bacteria under different air quality levels in Xinxiang, Central China. The results showed that as air quality deteriorated, the concentrations of airborne bacteria decreased while the relative amounts of pathogenic bacteria increased. Various environmental factors had different effects on the bacterial community structure and the presence of pathogenic bacteria. The deterioration of air quality inhibited the dissemination of ARGs.

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Determinants for antimicrobial resistance genes in farm dust on 333 poultry and pig farms in nine European countries

Roosmarijn E. C. Luiken et al.

Summary: This study quantified absolute and relative levels of antimicrobial resistance genes (ARGs) in airborne farm dust using qPCR. It found that farm dust is a large reservoir of ARGs, which can potentially transmit to bacteria in other reservoirs. The study also identified different pathways that contribute to shaping the dust resistome in livestock farms.

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Air pollution could drive global dissemination of antibiotic resistance genes

Guibing Zhu et al.

Summary: The study found widespread presence of antibiotic resistance genes (ARGs) in fresh snow, and their dissemination may be exacerbated by air pollution. Snowfall effectively spreads ARGs from point sources to the Earth's surface, highlighting the need for better pollution control measures to reduce the global dissemination risk of antibiotic resistance genes.

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Significant higher airborne antibiotic resistance genes and the associated inhalation risk in the indoor than the outdoor

Yanhui Zhao et al.

Summary: The study found that indoor air has significantly higher levels of ARGs and bacteria compared to outdoor air, with different airborne bacterial communities being the main drivers of variance in aerosol-associated ARGs. High concentrations of potential pathogenic bacteria and ARGs were found in indoor and outdoor aerosols, particularly indoors, posing an increased exposure risk through inhalation.

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S. Y. Jeong et al.

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Intracellular and Extracellular Antibiotic Resistance Genes in Airborne PM2.5 for Respiratory Exposure in Urban Areas

Tangtian He et al.

Summary: This study delves into the presence of intracellular and extracellular ARGs in the environment, finding that extracellular ARGs make up a significant proportion in PM2.5, with subtype-specific dominance in either free or phage-associated DNA. Variations in geographical locations due to regional meteorological factors and oxidative gases impact the distribution of PM2.5-associated intracellular and extracellular ARGs.

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Xiang Li et al.

Summary: Bacterial antibiotic resistance genes (ARGs) are prevalent and a serious issue in the air, yet there is limited understanding of ARG transmission in indoor air. The study found that air conditioner dust samples in hospitals exhibit a complex resistome, with the Outpatient hall identified as a main source of ARG transmission.

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Antimicrobial resistance genes are enriched in aerosols near impacted urban surface waters in La Paz, Bolivia

Olivia Ginn et al.

Summary: This study investigated the presence of antibiotic resistance genes (ARGs) and mobile integrons (MIs) in the urban air of La Paz, Bolivia. The study found that areas close to wastewater flows had higher concentrations of ARGs, while the density of ARGs decreased with increasing distance from impacted surface waters.

ENVIRONMENTAL RESEARCH (2021)

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Rainfall facilitates the transmission and proliferation of antibiotic resistance genes from ambient air to soil

Qing Wang et al.

Summary: This study investigates the role of rainfall in the transmission of ARGs, indicating that rainwater can facilitate the spread of ARGs, especially in soil contaminated with heavy metals.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

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Dispersion of Antibiotic Resistance Genes (ARGs) from stored swine manure biogas digestate to the atmosphere

Yu Zhang et al.

Summary: This study found that most of the ARG subtypes detected in biogas digestate could be dispersed into the atmosphere through aerosol dispersion, with different relative abundance and diversity of ARGs in biogas digestate compared to aerosols, and no significant correlation between the relative abundance of ARGs in biogas digestate and aerosols. Storage of biogas digestate led to an increase in abundance of certain ARG subtypes.

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Bioaerosol is an important transmission route of antibiotic resistance genes in pig farms

Lu Song et al.

Summary: The study reveals that bioaerosol is an important transmission route of antibiotic resistance genes in pig farms, with higher concentrations of ARGs and MGEs in winter compared to summer. It also found that wind speed promotes the spread of airborne ARGs, and higher temperatures favor a more diverse bacterial community.

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Spread of antibiotic resistance genes and microbiota in airborne particulate matter, dust, and human airways in the urban hospital

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